细胞生物学

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课程概况

细胞生物学是生命科学重要的基础学科之一，是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能，熟悉细胞生命活动的基本特点及其分子机制，并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学，即授课用中文，而PPT，教材及考试都为英文。适合对生物感兴趣，或者立志将来从事生命科学研究的学生进行学习。

课程大纲

1.Introduction of Molecular Cell Biology
1.1 Research objectives of the cell biology
1.2 Microscope and Cell theory
1.3 Major events in cell biology

2.Culturing,visualizing cells
2.1 Growing Cells in Culture
2.2 Monoclonal antibodies
2.3 Fluorescence Microscopy
2.4 Flow cytometry

3.Biomembrane structure
3.1 The structrure of plasmic membrane
3.2 The lipid bilayer:Composition and structural organization
3.3 Forms of lipid molecules exist in water
3.4 Lipids Movement in biomembrane
3.5 tructure of Membrane Proteins

4.Transmembrane transport of ions and small molecules
4.1 Overview of Transmembrane transport
42.Facilitated Transport of Glucose and Water
4.3 ATP –powered pumps
4.4 Na+/k+ pump
4.5 Ca2+ pump
4.6 K+ channel and the Resting membrane potencial
4.7 Cotransport by Symporters and Antiporters
4.8 Transcellular transport

5.Moving proteins into membrane and organells
5.1 Targeting Proteins to and Across the ER Membrane
5.2 Insertion of membrane Proteins into the ER
5.3 Protein Modifications,Folding,and Quality Control in the ER
5.4 Targeting of Proteins to Mitochondria and Chloroplasts
5.5 Targeting of Peroxisomal Proteins

6.Vesicular traffic,secretion and endocytosis
6.1 Molecular Mechanisms of Vesicle Budding and Fusion
6.2 Early Stages of the Secretory Pathway
6.3 Later Stages of the Secretory Pathway
6.4 The structure and functions of Lysosomes
6.5 Receptor-Mediated Endocytosis
6.6 Directing Membrane Proteins and Cytosolic Materials to the Lysosome
Midterm exam
7.Signal transduction
7.1 Brief Introduction of Cell Communication
7.2 Signaling Molecules and Receptors
7.3 G Proteins and G Protein-coupled Receptors (GPCR)
7.4 GPCR Mediated Signaling pathways

8.Signaling pathways that control gene activity
8.1 Protein Tyrosine Kinases (RTKs) and RTK-Ras Signaling Pathway
8.2 Phosphoinositide Signaling Pathways
8.3 TGF-b Receptors and TGFb Signaling Pathways
8.4 Cytokine receptors and JAK-STAT Signaling Pathways
8.5 Signaling Pathways controlled by Ubiquitination
8.6 Integration of Multiple Signaling Pathways

9: I.Microfilaments: Actin Structures
9.1 Actin Filaments
9.2 Dynamic Assembly of Actin Filaments
9.3 Actin-Based Cellular Structures
9.4 Myosins and Myosin-Powered Movements
9.5 Mechanisms of Cell Migration

10. II Microfilaments: Microtubules and Intermediate Filament
10.1 Structure and Organization of Microtubule
10.2 Regulation of Microtubule Dynamics
10.3 Microtubule-Based Motor Proteins
10.4 Intermediate Filaments
10.5 Coordination of Cellular Cytoskeleton

11.The Eukaryotic cell cycle
11.1. Overview of the cell cycle and its control
11.2. Model organisms and methods to study the cell cycle
11.3. CDKs and Regulation of CDK activity
11.4. Commitment to the cell cycle and DNA replication
11.5. Entry into mitosis
11.6. Completion of mitosis: chromosome segregation and mitotic exit
11.7. Surveillance mechanisms in cell cycle regulation

12.Integrating cells into tissues

13.Stem cells, cell asymmetry and cell death

14.Cancer
Final exam

预备知识

本课程的授课对象为大学二年级学生，前期课程应已修完生物化学、有机化学等基础学科。

证书或学分

本课程的学习过程包含：观看讲课视频、完成课后作业参加期中，期末考试。
课程学习的最终成绩由一下几个部分组成：
(1) 课后作业成绩占20%
(2)期中考试成绩占40%
(3) 期末考试成绩占40%
在第十三周进行期中考试，期中考试前的内容不再考察。
完成课程学习并考核合格(>=60分)的可获得合格证书，成绩优秀(>80分)的可获得优秀证书。

参考资料

Molecular Cell Biology, 7th Edition, Harvey Lodish/Arnold Berk, etc., 2013, Macmillan Higher Education
Molecular Biology of The Cell, 6th Edition, Alberts B., et al., John Wilson/Tim Hunt, 2015, Garland Science